Mario Bros. may refer to:
- Mario Bros.: The arcade game Mario Bros., and its remakes.
- Mario Bros. (stage): The stage in Super Smash Bros. Brawl.
- Mario Bros.: A music track playing in the same stage of Super Smash Bros. Brawl.
- Mario Bros. (team): The Mario/Luigi team, particularly in Super Smash Bros..
- Metal Bros.: Metallic versions of Mario & Luigi, a boss team in Super Smash Bros.