Damage that must be dealt to Assist Trophies to activate the yellow aura, at which point attacks which deal sufficient knockback will trigger a KO. The exact mechanisms behind how knockback calculations work on Assist Trophies and how much knockback must be dealt is currently unknown.
| Trophy | HP |
|---|---|
| Hammer Bro | 25 |
| Chain Chomp | 30 |
| Waluigi | 30 |
| Klaptrap | 40 |
| Midna | 50 |
| Ghirahim | 50 |
| Metroid | 21 |
| Mother Brain | 120 |
| Knuckle Joe | 25 |
| Chef Kawasaki | 80 |
| Krystal | 50 |
| Samurai Goroh | 25 |
| Starman | 40 |
| Tiki | 50 |
| Lyn | 23 |
| Black Knight | 70 |
| Phosphora | 25 |
| Snagret | 80 |
| Pong | 15 |
| Sheriff | 27 |
| Takamaru | 30 |
| Dr. Wright | 36 |
| Flies and Hand | 30 |
| Prince of Sablé | 35 |
| Sukapon | 50 |
| Isaac | 50 |
| Starfy | 35 |
| Dillon | 30 |
| Spring Man | 50 |
| Ghosts | 10 |
| Bomberman | 60 |
| Alucard | 50 |
| Wily Capsule | 45 |
| Zero | 60 |
| Knuckles | 45 |
| Guile | 60 |
| Akira | 60 |
| Gray Fox | 35 |
| Rathalos | 100 |
| Rodin | 55 |
| Shovel Knight | 45 |